I like to highlight some of the really interesting work we've been involved with, or that has come out of the Institute from time to time, and I recently updated our lab home page with links to a couple of papers. i thought I'd take the opportunity to write about them in a bit more detail here. Many of you will already know I run the Genomics Core facility at CRUKs Cambridge Institute. We do a lot of Illumina sequencing! The lab works on a huge number of projects for the research groups here in the Institute, and also across many groups in Cambridge via a long-running sequencing collaboration. We do do some R&D work in my lab, but >90% of our efforts are working with, or for, other research groups.
- Pereira et al Nature Communications 2016: The somatic mutation profiles of 2,433 breast cancers refine their genomic and transcriptomic landscapes
- Bruna et al Cell 2016: A Biobank of Breast Cancer Explants with Preserved Intra-tumor Heterogeneity to Screen Anticancer Compounds
- Lensing et al Nature Methods 2016: DSBCapture: in situ capture and sequencing of DNA breaks).
- Murtaza et al Nature Communications 2015: Multifocal clonal evolution characterized using circulating tumour DNA in a case of metastatic breast cancer
Highlights from the last years genomics research include work from the Caldas group who have completed three project over the lat year I've included here; 1) profiling of almost 2500 Breast Cancer patients for mutational analysis of 173 genes using a targeted pull-down (Pereira et al Nature Communications 2016); 2) cancer exomes from Murtaza et al,; 3) PDXs from Bruna et al.; and the Balasubramanian group who have shown that it is possible to capture and sequence double-strand DNA breaks (DSBs) in situ and directly map these at single-nucleotide resolution, enabling the study of DSB origin (Lensing et al. Nature Methods 2016). The rapid speed and unbiased nature of the genome-wide experiments being performed in the Institute, and often prepped and sequenced in the Genomics core continue to increase our understanding cancer biology.